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PD-L1 Kit ELISA

PD-L1 Reactivité: Souris Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
N° du produit ABIN5026968
  • Antigène Voir toutes PD-L1 Kits ELISA
    PD-L1 (CD274 (PD-L1))
    Reactivité
    • 9
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    • 2
    • 2
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    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156-10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the in vitro quantitative determination of Mouse PDL-1 concentrations in serum, plasma and other biological fluids.
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    < 0.094 ng/mL
    Ingrédients
    plate, standard, diluent, antibodies
    Matériel non inclus
    pipettes, tubes, reader, incubator
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  • Volume d'échantillon
    100 μL
    Durée du test
    3 - 4 h
    Plaque
    Pre-coated
    Protocole
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mouse PDL-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-MousePDL-1detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDL-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Mouse PDL-1 can be calculated using the standard curve.
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    protect your eyes
    Stock
    4 °C
  • Antigène Voir toutes PD-L1 Kits ELISA
    PD-L1 (CD274 (PD-L1))
    Autre désignation
    PD-L1 (PD-L1 Produits)
    Synonymes
    B7-H Kit ELISA, B7H1 Kit ELISA, PD-L1 Kit ELISA, PDCD1L1 Kit ELISA, PDCD1LG1 Kit ELISA, PDL1 Kit ELISA, A530045L16Rik Kit ELISA, B7h1 Kit ELISA, Pdcd1l1 Kit ELISA, Pdcd1lg1 Kit ELISA, Pdl1 Kit ELISA, RGD1566211 Kit ELISA, CD274 molecule Kit ELISA, CD274 antigen Kit ELISA, programmed cell death 1 ligand 2 Kit ELISA, CD274 Kit ELISA, Cd274 Kit ELISA, PDCD1LG2 Kit ELISA
    Pathways
    Cancer Immune Checkpoints
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