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HMGB1 Kit ELISA

HMGB1 Reactivité: Souris Colorimetric Sandwich ELISA Plasma, Serum, Tissue Homogenate
N° du produit ABIN5067936
  • Antigène Voir toutes HMGB1 Kits ELISA
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    Reactivité
    • 6
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    The Hmgb1 (High mobility group protein B1) ELISA Kit is a quantitative sandwich assay for the detection of Hmgb1 in mouse serum, plasma, tissue homogenates and other biological fluids.
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    9.375 pg/mL
    Attributs du produit
    Hmgb1 (High mobility group protein B1) ELISA Kit (Mouse)
    Ingrédients
    • *356299A: Microtiter Strips, 1x96 wells (8x12 wells).
    • *356299B: Standard, 2x1 vial
    • 356299C: Sample/Standard Dilution Buffer, 1x20ml
    • 356299D: Antibody (Biotin) (Concentrated), 1x120ul
    • 356299E: Antibody Dilution Buffer, 1x10ml
    • 356299F: Streptavidin (HRP) (SABC), 1x120ul
    • 356299G: SABC Dilution Buffer, 1x10ml
    • 356299H: TMB Substrate, 1x10ml
    • 356299J: Stop Solution, 1x10ml
    • 356299K: Wash Buffer, 25X, 1x30ml
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  • Plaque
    Pre-coated
    Protocole
    Principle:
    • This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to Hmgb1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hmgb1, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hmgb1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
    Précision du teste
    Precision:
    • Intra-assay CV: <8%
    • Inter-assay CV: <10%
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    4°C/-20°C
  • Antigène Voir toutes HMGB1 Kits ELISA
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    Autre désignation
    Hmgb1 (High mobility group protein B1) (HMGB1 Produits)
    Synonymes
    HMG1 Kit ELISA, HMG3 Kit ELISA, SBP-1 Kit ELISA, DEF Kit ELISA, HMG-1 Kit ELISA, Hmg1 Kit ELISA, amphoterin Kit ELISA, p30 Kit ELISA, hmgb1 Kit ELISA, ik:tdsubc_1a5 Kit ELISA, wu:fb23c02 Kit ELISA, xx:tdsubc_1a5 Kit ELISA, zgc:56110 Kit ELISA, zgc:77104 Kit ELISA, hmg-1 Kit ELISA, hmg3 Kit ELISA, sbp-1 Kit ELISA, hmg1 Kit ELISA, HMGB1 Kit ELISA, Ac2-008 Kit ELISA, high mobility group box 1 Kit ELISA, high-mobility group box 1 Kit ELISA, high mobility group box 1a Kit ELISA, high mobility group box 1 L homeolog Kit ELISA, high mobility group protein B1 Kit ELISA, HMGB1 Kit ELISA, Hmgb1 Kit ELISA, hmgb1 Kit ELISA, hmgb1a Kit ELISA, hmgb1.L Kit ELISA, LOC100359149 Kit ELISA
    Pathways
    Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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