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ULK1 Kit ELISA

ULK1 Reactivité: Humain, Souris pSer556 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN5526681
  • Antigène Voir toutes ULK1 Kits ELISA
    ULK1 (Unc-51 Like Kinase 1 (ULK1))
    Épitope
    pSer556, total
    Reactivité
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human and Mouse Phospho-ULK1 (S556) and Total ULK1 ELISA Kit. This assay semi-quantitatively measures ULK1 phosphorylated at Serine-556 as well as total ULK1 in cell lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human and Mouse ULK1 phosphorylated at site Serine-556 as well as total ULK1.
    Attributs du produit
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes ULK1 Kits ELISA
    ULK1 (Unc-51 Like Kinase 1 (ULK1))
    Autre désignation
    ULK1 (ULK1 Produits)
    Synonymes
    ATG1 Kit ELISA, ATG1A Kit ELISA, UNC51 Kit ELISA, Unc51.1 Kit ELISA, hATG1 Kit ELISA, si:dkey-11o18.7 Kit ELISA, ulk1 Kit ELISA, ULK1 Kit ELISA, AU041434 Kit ELISA, mKIAA0722 Kit ELISA, zgc:195008 Kit ELISA, unc-51 like autophagy activating kinase 1 Kit ELISA, unc-51 like kinase 1 Kit ELISA, unc-51 like autophagy activating kinase 1a Kit ELISA, ULK1 Kit ELISA, Ulk1 Kit ELISA, ulk1b Kit ELISA, ulk1a Kit ELISA
    ID gène
    8408
    UniProt
    O75385, O70405
    Pathways
    Regulation of Cell Size, Autophagy
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