Iba1 Kit CLIA

N° du produit ABIN5651596

Détail du produit Iba1 Kit CLIA

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Allograft Inflammatory Factor 1 (AIF1). No significant cross-reactivity or interference between Allograft Inflammatory Factor 1 (AIF1) and analogues was observed.

Sensibilité: 1.26 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Allograft Inflammatory Factor 1 (AIF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Allograft Inflammatory Factor 1 (AIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Allograft Inflammatory Factor 1 (AIF1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Allograft Inflammatory Factor 1 (AIF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Allograft Inflammatory Factor 1 (AIF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Autre désignation: Allograft Inflammatory Factor 1 (IBA1 Produits)

Synonymes: AIF-1 Kit CLIA, IBA1 Kit CLIA, IRT-1 Kit CLIA, IRT1 Kit CLIA, AI607846 Kit CLIA, D17H6S50E Kit CLIA, G1 Kit CLIA, Iba1 Kit CLIA, Bart1 Kit CLIA, iba1 Kit CLIA, mrf-1 Kit CLIA, fa04b11 Kit CLIA, wu:fa04b11 Kit CLIA, AIF Kit CLIA, AIF1 Kit CLIA, aif1 Kit CLIA, allograft inflammatory factor 1 Kit CLIA, AIF1 Kit CLIA, Aif1 Kit CLIA, aif1 Kit CLIA

Sujet:

Gene Name: Allograft Inflammatory Factor 1

Gene Aliases: IBA1, IRT1, Daintain, Protein G1, Ionized calcium-binding adapter molecule 1

ID gène: 11629

UniProt: O70200

Pathways: Smooth Muscle Cell Migration