SCNN1G Kit ELISA

N° du produit ABIN5651666

Détail du produit SCNN1G Kit ELISA

Antigène: SCNN1G (Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g). No significant cross-reactivity or interference between Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SCNN1G (Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G))

Autre désignation: Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1G Produits)

Synonymes: ENaC Kit ELISA, BESC3 Kit ELISA, ENaCg Kit ELISA, ENaCgamma Kit ELISA, PHA1 Kit ELISA, SCNEG Kit ELISA, scnn1g Kit ELISA, MGC81424 Kit ELISA, SCNN1G Kit ELISA, enacg Kit ELISA, gammaxENaC-A Kit ELISA, pha1 Kit ELISA, [g]ENaC Kit ELISA, GAMMA-ENAC Kit ELISA, gamma XENaC Kit ELISA, gamma-ENaC Kit ELISA, gamma2xENaC-A Kit ELISA, scneg Kit ELISA, sodium channel epithelial 1 gamma subunit Kit ELISA, sodium channel, nonvoltage-gated 1, gamma Kit ELISA, sodium channel, non voltage gated 1 gamma subunit L homeolog Kit ELISA, sodium channel, nonvoltage-gated 1 gamma Kit ELISA, sodium channel, non voltage gated 1 gamma subunit S homeolog Kit ELISA, Scnn1g Kit ELISA, SCNN1G Kit ELISA, scnn1g Kit ELISA, scnn1g.L Kit ELISA, scnn1g.S Kit ELISA

Sujet:

Gene Name: Amiloride Sensitive Sodium Channel Subunit Gamma

Gene Aliases: ENaCg, ENaCgamma, PHA1, SCNEG, Sodium Channel,Nonvoltage-Gated 1,Alpha, Epithelial Na(+) channel subunit gamma, Nonvoltage-gated sodium channel 1 subunit gamma

ID gène: 6340

UniProt: P51170