AGGF1 Kit ELISA

N° du produit ABIN5651723

Détail du produit AGGF1 Kit ELISA

Antigène: AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1 (AGGF1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.469 ng/mL - 30 ng/mL

Seuil minimal de détection: 0.469 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). No significant cross-reactivity or interference between Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) and analogues was observed.

Sensibilité: 0.19 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1 (AGGF1))

Autre désignation: Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1 Produits)

Synonymes: RGD1310888 Kit ELISA, AGGF1 Kit ELISA, LOC100037685 Kit ELISA, im:7146636 Kit ELISA, zgc:152959 Kit ELISA, DKFZp459G1317 Kit ELISA, GPATC7 Kit ELISA, GPATCH7 Kit ELISA, HSU84971 Kit ELISA, HUS84971 Kit ELISA, VG5Q Kit ELISA, 2010009L17Rik Kit ELISA, 2310029P06Rik Kit ELISA, AW112072 Kit ELISA, Peg3 Kit ELISA, angiogenic factor with G patch and FHA domains 1 Kit ELISA, angiogenic factor with G-patch and FHA domains 1 Kit ELISA, Aggf1 Kit ELISA, AGGF1 Kit ELISA, aggf1 Kit ELISA, CC1G_06398 Kit ELISA

Sujet:

Gene Name: Angiogenic Factor With G Patch And FHA Domains 1

Gene Aliases: GPATC7, GPATCH7, VG5Q, Angiogenic factor VG5Q, G patch domain-containing protein 7, Vasculogenesis gene on 5q protein