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Angiotensin II Type-1 Receptor Kit ELISA

AGTR1 Reactivité: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5651799
  • Antigène Voir toutes Angiotensin II Type-1 Receptor (AGTR1) Kits ELISA
    Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))
    Reactivité
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    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Angiotensin II Receptor 1 (AGTR1). No significant cross-reactivity or interference between Angiotensin II Receptor 1 (AGTR1) and analogues was observed.
    Sensibilité
    0.053 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II Receptor 1 (AGTR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin II Receptor 1 (AGTR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin II Receptor 1 (AGTR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin II Receptor 1 (AGTR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II Receptor 1 (AGTR1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II Receptor 1 (AGTR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Angiotensin II Type-1 Receptor (AGTR1) Kits ELISA
    Angiotensin II Type-1 Receptor (AGTR1) (Angiotensin II Receptor, Type 1 (AGTR1))
    Autre désignation
    Angiotensin II Receptor 1 (AGTR1 Produits)
    Synonymes
    AG2S Kit ELISA, AGTR1A Kit ELISA, AGTR1B Kit ELISA, AT1 Kit ELISA, AT1AR Kit ELISA, AT1B Kit ELISA, AT1BR Kit ELISA, AT1R Kit ELISA, AT2R1 Kit ELISA, AT2R1A Kit ELISA, AT2R1B Kit ELISA, HAT1R Kit ELISA, 1810074K20Rik Kit ELISA, AI551199 Kit ELISA, AT1a Kit ELISA, Agtr-1a Kit ELISA, Agtr1 Kit ELISA, Angtr-1a Kit ELISA, AT1A Kit ELISA, XAT-1 Kit ELISA, agtr1-A Kit ELISA, agtr1.2 Kit ELISA, AT1-R Kit ELISA, at1 Kit ELISA, AGTR1 Kit ELISA, Agtr-1b Kit ELISA, Angtr-1b Kit ELISA, agtr1 Kit ELISA, agtr1-a Kit ELISA, agtr1-b Kit ELISA, xAT Kit ELISA, angiotensin II receptor type 1 Kit ELISA, angiotensin II receptor, type 1a Kit ELISA, angiotensin II receptor type 1 S homeolog Kit ELISA, uncharacterized AGTR1 Kit ELISA, angiotensin II receptor, type 1b Kit ELISA, angiotensin II receptor type 1 L homeolog Kit ELISA, AGTR1 Kit ELISA, Agtr1a Kit ELISA, agtr1.S Kit ELISA, Agtr1 Kit ELISA, Agtr1b Kit ELISA, agtr1.L Kit ELISA
    Sujet

    Gene Name: Angiotensin II Receptor 1

    Gene Aliases: AT1R, AG2S, AG2-S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2-R1, AT2R1A, AT2R1B, HAT1R, AT1 Receptor, Angiotensin Receptor 1B

    ID gène
    24180
    UniProt
    P25095
    Pathways
    Signalistation JAK/STAT, ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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