APAF1 Kit CLIA

N° du produit ABIN5651961

Détail du produit APAF1 Kit CLIA

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1). No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.

Sensibilité: 6.27 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apoptotic Peptidase Activating Factor 1 (APAF1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apoptotic Peptidase Activating Factor 1 (APAF1 Produits)

Synonymes: APAF1 Kit CLIA, ARK Kit CLIA, Apaf-1 Kit CLIA, Apaf1 Kit CLIA, CG6829 Kit CLIA, D-Apaf-1 Kit CLIA, DARK Kit CLIA, Dapaf-1 Kit CLIA, Dapaf-1/HAC-1 Kit CLIA, Dark Kit CLIA, Dark/Apaf-I Kit CLIA, Dark/Dapaf-1/HAC1 Kit CLIA, Dark/Hac-1/dApaf-1 Kit CLIA, Dark/Hac-1/dApaf1 Kit CLIA, Dmel\\CG6829 Kit CLIA, Hac-1 Kit CLIA, Hac-1/Dark Kit CLIA, Hac1 Kit CLIA, T1 Kit CLIA, anon-53Fa Kit CLIA, apaf-1 Kit CLIA, apaf1 Kit CLIA, arc Kit CLIA, ark Kit CLIA, dAPAF-1 Kit CLIA, dApaf-1 Kit CLIA, dApaf-1/DARK/HAC-1 Kit CLIA, dApaf1 Kit CLIA, dArk Kit CLIA, dapaf Kit CLIA, dapaf-1 Kit CLIA, dapaf-1L Kit CLIA, dapaf-1S Kit CLIA, dark Kit CLIA, dark/dapaf-1/hac-1 Kit CLIA, dark/hac-1/dapaf-1 Kit CLIA, hac-1 Kit CLIA, hac1 Kit CLIA, l(2)SH0173 Kit CLIA, APAF-1 Kit CLIA, CED4 Kit CLIA, 6230400I06Rik Kit CLIA, Apaf1l Kit CLIA, fog Kit CLIA, mKIAA0413 Kit CLIA, zgc:136543 Kit CLIA, Death-associated APAF1-related killer Kit CLIA, apoptotic peptidase activating factor 1 L homeolog Kit CLIA, apoptotic peptidase activating factor 1 Kit CLIA, Dark Kit CLIA, apaf1.L Kit CLIA, apaf1 Kit CLIA, APAF1 Kit CLIA, Apaf1 Kit CLIA

Sujet:

Gene Name: Apoptotic Peptidase Activating Factor 1

Gene Aliases: CED4, Apoptotic Protease Activating Factor

ID gène: 317

UniProt: O14727

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity