ABCC2 Kit ELISA
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- Antigène Voir toutes ABCC2 Kits ELISA
- ABCC2 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2))
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C2 (ABCC2). No significant cross-reactivity or interference between ATP Binding Cassette Transporter C2 (ABCC2) and analogues was observed.
- Sensibilité
- 0.067 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C2 (ABCC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C2 (ABCC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C2 (ABCC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C2 (ABCC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C2 (ABCC2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C2 (ABCC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes ABCC2 Kits ELISA
- ABCC2 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2))
- Autre désignation
- ATP Binding Cassette Transporter C2 (ABCC2 Produits)
- Synonymes
- ABC30 Kit ELISA, CMOAT Kit ELISA, DJS Kit ELISA, MRP2 Kit ELISA, cMRP Kit ELISA, AI173996 Kit ELISA, Abc30 Kit ELISA, Cmoat Kit ELISA, Mrp2 Kit ELISA, ATP binding cassette subfamily C member 2 Kit ELISA, ATP-binding cassette, sub-family C (CFTR/MRP), member 2 Kit ELISA, ABCC2 Kit ELISA, Abcc2 Kit ELISA
- Sujet
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Gene Name: ATP Binding Cassette Transporter C2
Gene Aliases: CMOAT1, ABC30, CMOAT, DJS, MRP2, cMRP, Canalicular Multispecific Organic Anion transporter 1, Canalicular multidrug resistance protein, Multidrug resistance-associated 2
- ID gène
- 1244
- UniProt
- Q92887
- Pathways
- Hormone Transport
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