ABCC8 Kit ELISA

N° du produit ABIN5652076

Détail du produit ABCC8 Kit ELISA

Antigène: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.25 ng/mL - 20 ng/mL

Seuil minimal de détection: 1.25 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C8 (ABCC8). No significant cross-reactivity or interference between ATP Binding Cassette Transporter C8 (ABCC8) and analogues was observed.

Sensibilité: 0.52 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C8 (ABCC8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C8 (ABCC8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C8 (ABCC8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C8 (ABCC8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C8 (ABCC8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C8 (ABCC8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Autre désignation: ATP Binding Cassette Transporter C8 (ABCC8 Produits)

Synonymes: ABC36 Kit ELISA, HHF1 Kit ELISA, HI Kit ELISA, HRINS Kit ELISA, MRP8 Kit ELISA, PHHI Kit ELISA, SUR Kit ELISA, SUR1 Kit ELISA, SUR1delta2 Kit ELISA, TNDM2 Kit ELISA, D930031B21Rik Kit ELISA, Sur Kit ELISA, Sur1 Kit ELISA, ATP binding cassette subfamily C member 8 Kit ELISA, ATP-binding cassette, sub-family C (CFTR/MRP), member 8 Kit ELISA, ABCC8 Kit ELISA, Abcc8 Kit ELISA

Sujet:

Gene Name: ATP Binding Cassette Transporter C8

Gene Aliases: ABC-C8, PHHI, MRP8, ABC36, HHF1, HI, HRINS, SUR, SUR1, TNDM2, Sulfonylurea Receptor(Hyperinsulinemia)

ID gène: 6833

UniProt: Q09428

Pathways: Negative Regulation of Hormone Secretion