ATP2A2 Kit ELISA
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- Antigène Voir toutes ATP2A2 Kits ELISA
- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed.
- Sensibilité
- 0.055 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes ATP2A2 Kits ELISA
- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
- Autre désignation
- ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2 Produits)
- Synonymes
- atp2b Kit ELISA, ca-p60a Kit ELISA, dar Kit ELISA, serca2 Kit ELISA, ATP2A2 Kit ELISA, ATP2B Kit ELISA, DAR Kit ELISA, DD Kit ELISA, SERCA2 Kit ELISA, SERCA2A Kit ELISA, ATP2 Kit ELISA, Serca2 Kit ELISA, SercaII Kit ELISA, 9530097L16Rik Kit ELISA, D5Wsu150e Kit ELISA, SERCA2B Kit ELISA, mKIAA4195 Kit ELISA, atp2a2 Kit ELISA, zgc:55380 Kit ELISA, ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 S homeolog Kit ELISA, ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 Kit ELISA, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 Kit ELISA, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a Kit ELISA, atp2a1.S Kit ELISA, ATP2A2 Kit ELISA, Atp2a2 Kit ELISA, atp2a2a Kit ELISA
- Sujet
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Gene Name: ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2
Gene Aliases: ATP2B, DAR, SERCA2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle
- Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process
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