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ATP6AP2 Kit ELISA

ATP6AP2 Reactivité: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5652090
  • Antigène Voir toutes ATP6AP2 Kits ELISA
    ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))
    Reactivité
    • 2
    • 2
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). No significant cross-reactivity or interference between ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) and analogues was observed.
    Sensibilité
    0.128 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes ATP6AP2 Kits ELISA
    ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))
    Autre désignation
    ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2 Produits)
    Synonymes
    APT6M8-9 Kit ELISA, ATP6IP2 Kit ELISA, ATP6M8-9 Kit ELISA, ELDF10 Kit ELISA, M8-9 Kit ELISA, MRXE Kit ELISA, MSTP009 Kit ELISA, XMRE Kit ELISA, (P)RR Kit ELISA, 5730403E06Rik Kit ELISA, Atp6ip2 Kit ELISA, tj6 Kit ELISA, wss Kit ELISA, arcl Kit ELISA, j6b7 Kit ELISA, stv1 Kit ELISA, tj6m Kit ELISA, tj6s Kit ELISA, vph1 Kit ELISA, atp6a2 Kit ELISA, atp6n1d Kit ELISA, ATP6AP2 Kit ELISA, DKFZp459G0918 Kit ELISA, atp6ap2 Kit ELISA, wu:fj36c03 Kit ELISA, wu:fq15e04 Kit ELISA, zgc:73194 Kit ELISA, ATPase H+ transporting accessory protein 2 Kit ELISA, ATPase, H+ transporting, lysosomal accessory protein 2 Kit ELISA, ATPase H+ transporting V0 subunit d2 Kit ELISA, ATPase H+ transporting V0 subunit a2 Kit ELISA, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog Kit ELISA, ATP6AP2 Kit ELISA, Atp6ap2 Kit ELISA, ATP6V0D2 Kit ELISA, atp6v0a2 Kit ELISA, atp6ap2.S Kit ELISA, atp6ap2 Kit ELISA
    Sujet

    Gene Name: ATPase, H+ Transporting, Lysosomal Accessory Protein 2

    Gene Aliases: ATP6IP2, CAPER, ELDF10, XMRE, Renin/prorenin receptor, Embryonic liver differentiation factor 10, Vacuolar ATP synthase membrane sector-associated protein M8-9

    ID gène
    302526
    UniProt
    Q6AXS4
    Pathways
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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