Bcl-2 Kit CLIA

N° du produit ABIN5652139

Détail du produit Bcl-2 Kit CLIA

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 4.69 ng/mL - 300 ng/mL

Seuil minimal de détection: 4.69 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of B-Cell Leukemia/Lymphoma 2 (Bcl2). No significant cross-reactivity or interference between B-Cell Leukemia/Lymphoma 2 (Bcl2) and analogues was observed.

Sensibilité: 0.17 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the B-Cell Leukemia/Lymphoma 2 (Bcl2) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: B-Cell Leukemia/Lymphoma 2 (BCL2 Produits)

Synonymes: Bcl-2 Kit CLIA, PPP1R50 Kit CLIA, bcl-2 Kit CLIA, AW986256 Kit CLIA, C430015F12Rik Kit CLIA, D630044D05Rik Kit CLIA, D830018M01Rik Kit CLIA, BCL-2 Kit CLIA, Bcl2 Kit CLIA, BCL2, apoptosis regulator Kit CLIA, B cell leukemia/lymphoma 2 Kit CLIA, B-cell CLL/lymphoma 2a Kit CLIA, B-cell CLL/lymphoma 2 protein Kit CLIA, BCL2 Kit CLIA, Bcl2 Kit CLIA, bcl2a Kit CLIA, BCL-2 Kit CLIA

Sujet:

Gene Name: B-Cell Leukemia/Lymphoma 2

Gene Aliases: B-Cell CLL/Lymphoma 2

ID gène: 596

UniProt: P10415

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling