CACNA1C Kit ELISA

N° du produit ABIN5652483

Détail du produit CACNA1C Kit ELISA

Antigène: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Autre désignation: Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNA1C Produits)

Synonymes: CACH2 Kit ELISA, CACN2 Kit ELISA, CACNL1A1 Kit ELISA, CCHL1A1 Kit ELISA, CaV1.2 Kit ELISA, LQT8 Kit ELISA, TS Kit ELISA, Cav1.2 Kit ELISA, Cchl1a1 Kit ELISA, MBC Kit ELISA, MELC-CC Kit ELISA, RATIVS302 Kit ELISA, isl Kit ELISA, C-LTCC Kit ELISA, CHCACHA1C Kit ELISA, ICA Kit ELISA, cav1.2 Kit ELISA, ca(v)1.2 Kit ELISA, CAV1.2 Kit ELISA, BCR Kit ELISA, rbc Kit ELISA, calcium voltage-gated channel subunit alpha1 C Kit ELISA, calcium channel, voltage-dependent, L type, alpha 1C subunit Kit ELISA, voltage-dependent L-type calcium channel subunit alpha-1C Kit ELISA, calcium channel, voltage-dependent, L type, alpha 1C subunit S homeolog Kit ELISA, Red blood cell count Kit ELISA, RNA, 7SL, cytoplasmic 263, pseudogene Kit ELISA, CACNA1C Kit ELISA, Cacna1c Kit ELISA, cacna1c Kit ELISA, LOC100442085 Kit ELISA, cacna1c.S Kit ELISA, RBC Kit ELISA, RN7SL263P Kit ELISA

Sujet:

Gene Name: Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit

Gene Aliases: CACH2, CACN2, CACNL1A1, CCHL1A1, CaV1.2, TS, Voltage-gated calcium channel subunit alpha Cav1.2, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle

ID gène: 12288

UniProt: Q01815

Pathways: Hormone Transport, Carbohydrate Homeostasis