CAMKK2 Kit ELISA

N° du produit ABIN5652500

Détail du produit CAMKK2 Kit ELISA

Antigène: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Autre désignation: Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2 Produits)

Synonymes: CAMKK Kit ELISA, CAMKKB Kit ELISA, 6330570N16Rik Kit ELISA, AW061083 Kit ELISA, mKIAA0787 Kit ELISA, camk2b Kit ELISA, fj90d04 Kit ELISA, wu:fj90d04 Kit ELISA, si:dkey-174n20.2 Kit ELISA, CAMKK2 Kit ELISA, calcium/calmodulin dependent protein kinase kinase 2 Kit ELISA, calcium/calmodulin-dependent protein kinase kinase 2, beta Kit ELISA, calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 1 Kit ELISA, calcium/calmodulin dependent protein kinase II beta Kit ELISA, calcium/calmodulin-dependent protein kinase kinase 2 Kit ELISA, CAMKK2 Kit ELISA, Camkk2 Kit ELISA, camk2b1 Kit ELISA, CAMK2B Kit ELISA, camkk2 Kit ELISA

Sujet:

Gene Name: Calcium/Calmodulin Dependent Protein Kinase Kinase 2

Gene Aliases: CAMKK, CAMKKB, Calcium/calmodulin-dependent protein kinase kinase beta

Pathways: AMPK Signaling