SLC25A20 Kit ELISA

N° du produit ABIN5652611

Détail du produit SLC25A20 Kit ELISA

Antigène: SLC25A20 (Solute Carrier Family 25 (Carnitine/acylcarnitine Translocase), Member 20 (SLC25A20))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Carnitine Acylcarnitine Translocase (CACT). No significant cross-reactivity or interference between Carnitine Acylcarnitine Translocase (CACT) and analogues was observed.

Sensibilité: 0.133 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acylcarnitine Translocase (CACT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Acylcarnitine Translocase (CACT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Acylcarnitine Translocase (CACT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Acylcarnitine Translocase (CACT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Acylcarnitine Translocase (CACT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Acylcarnitine Translocase (CACT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC25A20 (Solute Carrier Family 25 (Carnitine/acylcarnitine Translocase), Member 20 (SLC25A20))

Autre désignation: Carnitine Acylcarnitine Translocase (SLC25A20 Produits)

Synonymes: 5848 Kit ELISA, BG:DS02740.15 Kit ELISA, CACT Kit ELISA, CG5848 Kit ELISA, Cact Kit ELISA, Dmel\\CG5848 Kit ELISA, cac Kit ELISA, dip6 Kit ELISA, fs(2)ltoRN48 Kit ELISA, n(2)k17003 Kit ELISA, cact Kit ELISA, dif-1 Kit ELISA, SLC25A20 Kit ELISA, DKFZp468F1219 Kit ELISA, zgc:77760 Kit ELISA, PRKAR2A Kit ELISA, CAC Kit ELISA, 1110007P09Rik Kit ELISA, C78826 Kit ELISA, mCAC Kit ELISA, cactus Kit ELISA, solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 Kit ELISA, solute carrier family 25 member 20 Kit ELISA, solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 L homeolog Kit ELISA, solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20 Kit ELISA, cact Kit ELISA, slc25a20 Kit ELISA, SLC25A20 Kit ELISA, Slc25a20 Kit ELISA, slc25a20.L Kit ELISA

Sujet:

Gene Name: Carnitine Acylcarnitine Translocase

Gene Aliases: SLC25A20, CAC, Solute Carrier Family 25 Member 20, Carnitine Acylcarnitine Carrier, Mitochondrial carnitine/acylcarnitine carrier protein

UniProt: P97521

Pathways: TCR Signaling, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades