Caspase 8 Kit ELISA

N° du produit ABIN5652663

Détail du produit Caspase 8 Kit ELISA

Antigène: Caspase 8 (CASP8)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Caspase 8 (CASP8). No significant cross-reactivity or interference between Caspase 8 (CASP8) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 (CASP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 (CASP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 (CASP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 8 (CASP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Caspase 8 (CASP8)

Autre désignation: Caspase 8 (CASP8 Produits)

Synonymes: casp8-A Kit ELISA, xCaspase-8 Kit ELISA, casp8 Kit ELISA, CASP8 Kit ELISA, CASP-8 Kit ELISA, FLICE Kit ELISA, MACH Kit ELISA, Mch5 Kit ELISA, ALPS2B Kit ELISA, CAP4 Kit ELISA, Casp-8 Kit ELISA, MCH5 Kit ELISA, caspase-8 Kit ELISA, zgc:92075 Kit ELISA, caspase 8 L homeolog Kit ELISA, death related ced-3/Nedd2-like protein Kit ELISA, caspase 8 Kit ELISA, caspase-8 Kit ELISA, caspase-8-like Kit ELISA, caspase 8, apoptosis-related cysteine peptidase Kit ELISA, casp8.L Kit ELISA, LOC661095 Kit ELISA, CASP8 Kit ELISA, LOC5563818 Kit ELISA, casp8 Kit ELISA, LOC100222284 Kit ELISA, Casp8 Kit ELISA

Sujet:

Gene Name: Caspase 8

Gene Aliases: CAP4, FLICE, MACH, MCH5, Cysteinyl Aspartate Specific Proteinases 8, Apoptotic cysteine protease, FADD-homologous ICE/ced-3-like protease, MORT1-associated ced-3 homolog

ID gène: 64044

UniProt: Q9JHX4

Pathways: Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades