Cathepsin D Kit CLIA
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- Antigène Voir toutes Cathepsin D (CTSD) Kits CLIA
- Cathepsin D (CTSD)
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Reactivité
- Souris
- Méthode de détection
- Chemiluminescent
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.25 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.25 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD). No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.
- Sensibilité
- 0.98 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 - 3 h
- Plaque
- Pre-coated
- Protocole
- The microplate provided in this kit has been pre-coated with an antibody specific to Cathepsin D (CTSD). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cathepsin D (CTSD) level in the sample or standard.,
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Cathepsin D (CTSD) Kits CLIA
- Cathepsin D (CTSD)
- Autre désignation
- Cathepsin D (CTSD Produits)
- Synonymes
- catD Kit CLIA, fb93e11 Kit CLIA, fj17b09 Kit CLIA, wu:fb93e11 Kit CLIA, wu:fj17b09 Kit CLIA, LOC398557 Kit CLIA, Cathd Kit CLIA, CatD Kit CLIA, LOC100136761 Kit CLIA, CTSD Kit CLIA, DKFZp469J0315 Kit CLIA, DDBDRAFT_0205785 Kit CLIA, DDBDRAFT_0215012 Kit CLIA, DDB_0205785 Kit CLIA, DDB_0215012 Kit CLIA, CLN10 Kit CLIA, CPSD Kit CLIA, CD Kit CLIA, cathepsin D Kit CLIA, cathepsin D S homeolog Kit CLIA, lysosomal aspartic protease Kit CLIA, cathepsin D-like Kit CLIA, ctsd Kit CLIA, LOC398557 Kit CLIA, ctsd.S Kit CLIA, CatD Kit CLIA, LOC5567565 Kit CLIA, LOC100136761 Kit CLIA, CTSD Kit CLIA, Cathd Kit CLIA, ctsD Kit CLIA, catd Kit CLIA, Ctsd Kit CLIA, LOC443060 Kit CLIA
- Sujet
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Gene Name: Cathepsin D
Gene Aliases: CPSD, CLN10, Lysosomal Aspartyl Protease, Ceroid-Lipofuscinosis,Neuronal 10
- ID gène
- 13033
- UniProt
- P18242
- Pathways
- Peptide Hormone Metabolism
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