COL4A1 Kit ELISA

N° du produit ABIN5653128

Détail du produit COL4A1 Kit ELISA

Antigène: COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.69 ng/mL - 300 ng/mL

Seuil minimal de détection: 4.69 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 1 (COL4a1). No significant cross-reactivity or interference between Collagen Type IV Alpha 1 (COL4a1) and analogues was observed.

Sensibilité: 1.8 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 1 (COL4a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV Alpha 1 (COL4a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 1 (COL4a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV Alpha 1 (COL4a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))

Autre désignation: Collagen Type IV Alpha 1 (COL4A1 Produits)

Synonymes: col4a1 Kit ELISA, wu:fc02g11 Kit ELISA, HANAC Kit ELISA, ICH Kit ELISA, POREN1 Kit ELISA, arresten Kit ELISA, Bru Kit ELISA, Col4a-1 Kit ELISA, Del(8)44H Kit ELISA, Raw Kit ELISA, Svc Kit ELISA, collagen, type IV, alpha 5 (Alport syndrome) Kit ELISA, collagen type IV alpha 1 chain Kit ELISA, collagen, type IV, alpha 1 Kit ELISA, col4a5 Kit ELISA, COL4A1 Kit ELISA, Col4a1 Kit ELISA

Sujet:

Gene Name: Collagen Type IV Alpha 1

Gene Aliases: COL4-A1, Arresten, Collagen Alpha-1(IV)chain

ID gène: 1282

UniProt: P02462

Pathways: Skeletal Muscle Fiber Development, Growth Factor Binding