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SERPING1 Kit ELISA

SERPING1 Reactivité: Souris Colorimetric Sandwich ELISA 15.62 ng/mL - 1000 ng/mL Plasma, Serum
N° du produit ABIN5653191
  • Antigène Voir toutes SERPING1 Kits ELISA
    SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))
    Reactivité
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.62 ng/mL - 1000 ng/mL
    Seuil minimal de détection
    15.62 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Complement 1 Inhibitor (C1INH). No significant cross-reactivity or interference between Complement 1 Inhibitor (C1INH) and analogues was observed.
    Sensibilité
    5.9 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1 Inhibitor (C1INH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement 1 Inhibitor (C1INH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1 Inhibitor (C1INH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement 1 Inhibitor (C1INH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1 Inhibitor (C1INH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes SERPING1 Kits ELISA
    SERPING1 (Serpin Peptidase Inhibitor, Clade G (C1 Inhibitor), Member 1 (SERPING1))
    Autre désignation
    Complement 1 Inhibitor (SERPING1 Produits)
    Synonymes
    fb64f02 Kit ELISA, im:7159527 Kit ELISA, si:dkeyp-110g5.2 Kit ELISA, wu:fb60c09 Kit ELISA, wu:fb62a08 Kit ELISA, wu:fb64f02 Kit ELISA, LOC100136072 Kit ELISA, C1-INH Kit ELISA, C1INH Kit ELISA, C1nh Kit ELISA, C1IN Kit ELISA, C1NH Kit ELISA, HAE1 Kit ELISA, HAE2 Kit ELISA, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 Kit ELISA, C1 inhibitor Kit ELISA, serpin family G member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade G, member 1 Kit ELISA, serping1 Kit ELISA, LOC100136072 Kit ELISA, SERPING1 Kit ELISA, Serping1 Kit ELISA
    Sujet

    Gene Name: Complement 1 Inhibitor

    Gene Aliases: SERPING1, C1IN, C1-INH, C1NH, HAE1, HAE2, Serpin Peptidase Inhibitor Clade G Member 1, Angioedema Hereditary, C1 Inhibitor, C1 Esterase Inhibitor

    UniProt
    P97290
    Pathways
    Système du Complément
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