ENPP1 Kit ELISA

N° du produit ABIN5653779

Détail du produit ENPP1 Kit ELISA

Antigène: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). No significant cross-reactivity or interference between Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Autre désignation: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1 Produits)

Synonymes: ENPP1 Kit ELISA, si:ch211-142e24.2 Kit ELISA, zgc:136238 Kit ELISA, ARHR2 Kit ELISA, M6S1 Kit ELISA, NPP1 Kit ELISA, NPPS Kit ELISA, PC-1 Kit ELISA, PCA1 Kit ELISA, PDNP1 Kit ELISA, 4833416E15Rik Kit ELISA, AI428932 Kit ELISA, C76301 Kit ELISA, CD203c Kit ELISA, E-NPP1 Kit ELISA, Ly-41 Kit ELISA, Npps Kit ELISA, Pca Kit ELISA, Pca-1 Kit ELISA, Pdnp1 Kit ELISA, ttw Kit ELISA, twy Kit ELISA, Pc1 Kit ELISA, ectonucleotide pyrophosphatase/phosphodiesterase 1 Kit ELISA, ectonucleotide pyrophosphatase/phosphodiesterase 1 L homeolog Kit ELISA, ENPP1 Kit ELISA, enpp1 Kit ELISA, enpp1.L Kit ELISA, Enpp1 Kit ELISA

Sujet:

Gene Name: Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Gene Aliases: M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, Membrane component chromosome 6 surface marker 1, Alkaline phosphodiesterase I, Plasma-cell membrane glycoprotein PC-1

ID gène: 5167

UniProt: P22413

Pathways: Regulation of Carbohydrate Metabolic Process