ESR2 Kit ELISA

N° du produit ABIN5653991

Détail du produit ESR2 Kit ELISA

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Autre désignation: Estrogen Receptor Beta (ESR2 Produits)

Synonymes: ER-BETA Kit ELISA, ESR-BETA Kit ELISA, ESRB Kit ELISA, ESTRB Kit ELISA, Erb Kit ELISA, NR3A2 Kit ELISA, ER[b]1 Kit ELISA, zfER-beta1 Kit ELISA, zfER[b]1 Kit ELISA, erb2 Kit ELISA, CERB Kit ELISA, ERB Kit ELISA, erb Kit ELISA, esrb Kit ELISA, estrb Kit ELISA, nr3a2 Kit ELISA, ERbeta Kit ELISA, er-beta Kit ELISA, esr-beta Kit ELISA, ESR2 Kit ELISA, ERBETA Kit ELISA, er Kit ELISA, ER-beta Kit ELISA, ER[b] Kit ELISA, Estrb Kit ELISA, ER[b]2 Kit ELISA, ER[b]a Kit ELISA, zfER[b]2 Kit ELISA, zfER-beta2 Kit ELISA, Erb2 Kit ELISA, esr2 Kit ELISA, erbeta Kit ELISA, Esr2 Kit ELISA, ERBeta2 Kit ELISA, estrogen receptor 2 Kit ELISA, estrogen receptor 2b Kit ELISA, estrogen receptor beta 2 Kit ELISA, estrogen receptor 2 (beta) Kit ELISA, estrogen receptor 2a Kit ELISA, estrogen receptor beta Kit ELISA, ESR2 Kit ELISA, esr2b Kit ELISA, erb2 Kit ELISA, esr2 Kit ELISA, Esr2 Kit ELISA, esr2a Kit ELISA, erbeta Kit ELISA

Sujet:

Gene Name: Estrogen Receptor Beta

Gene Aliases: ER-B, ESRB, ESTRB, NR3A2, NR3-A2, ESR2, Estrogen Receptor 2, Nuclear Receptor Subfamily 3,Group A,Member 2

ID gène: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling