SLC1A2 Kit ELISA

N° du produit ABIN5654012

Détail du produit SLC1A2 Kit ELISA

Antigène: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 2 (EAAT2). No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 2 (EAAT2) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 2 (EAAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 2 (EAAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Autre désignation: Excitatory Amino Acid Transporter 2 (SLC1A2 Produits)

Synonymes: CG3159 Kit ELISA, Dmel\\CG3159 Kit ELISA, EAAT Kit ELISA, dEAAT2 Kit ELISA, dEaat2 Kit ELISA, Eaat Kit ELISA, GB16377 Kit ELISA, EAAT2 Kit ELISA, SLC1A2 Kit ELISA, Eaat2 Kit ELISA, Glt Kit ELISA, Glt-1 Kit ELISA, GLT-1 Kit ELISA, 1700091C19Rik Kit ELISA, 2900019G14Rik Kit ELISA, AI159670 Kit ELISA, GLT1 Kit ELISA, MGLT1 Kit ELISA, eaat2 Kit ELISA, fj34b12 Kit ELISA, slc1a2 Kit ELISA, wu:fj34b12 Kit ELISA, zgc:65897 Kit ELISA, Excitatory amino acid transporter 2 Kit ELISA, excitatory amino acid transporter 2 Kit ELISA, solute carrier family 1 member 2 Kit ELISA, solute carrier family 1 (glial high affinity glutamate transporter), member 2 Kit ELISA, solute carrier family 1 (glial high affinity glutamate transporter), member 2b Kit ELISA, Eaat2 Kit ELISA, Eaat-2 Kit ELISA, SLC1A2 Kit ELISA, VDBG_06180 Kit ELISA, EUBELI_01747 Kit ELISA, Trebr_0139 Kit ELISA, Slc1a2 Kit ELISA, slc1a2b Kit ELISA

Sujet:

Gene Name: Excitatory Amino Acid Transporter 2

Gene Aliases: SLC1A2, EAAT2, GLT-1, Solute Carrier Family 1 Member 2, Glutamate/aspartate transporter II, Sodium-dependent glutamate/aspartate transporter 2

Pathways: Dicarboxylic Acid Transport