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SLC1A1 Kit ELISA

SLC1A1 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5654014
  • Antigène Voir toutes SLC1A1 Kits ELISA
    SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Excitatory Amino Acid Transporter 3 (EAAT3). No significant cross-reactivity or interference between Excitatory Amino Acid Transporter 3 (EAAT3) and analogues was observed.
    Sensibilité
    0.117 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 3 (EAAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 3 (EAAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 3 (EAAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excitatory Amino Acid Transporter 3 (EAAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 3 (EAAT3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 3 (EAAT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes SLC1A1 Kits ELISA
    SLC1A1 (Solute Carrier Family 1 (Neuronal/epithelial High Affinity Glutamate Transporter, System Xag), Member 1 (SLC1A1))
    Autre désignation
    Excitatory Amino Acid Transporter 3 (SLC1A1 Produits)
    Synonymes
    GB16911 Kit ELISA, EAAT3 Kit ELISA, SLC1A2a Kit ELISA, zgc:91959 Kit ELISA, EAAC1 Kit ELISA, SCZD18 Kit ELISA, D130048G10Rik Kit ELISA, EAAC2 Kit ELISA, MEAAC1 Kit ELISA, Eaac1 Kit ELISA, Eaat3 Kit ELISA, REAAC1 Kit ELISA, excitatory amino acid transporter 3 Kit ELISA, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 Kit ELISA, solute carrier family 1 member 1 Kit ELISA, LOC412382 Kit ELISA, slc1a1 Kit ELISA, SLC1A1 Kit ELISA, CpipJ_CPIJ000674 Kit ELISA, CpipJ_CPIJ001134 Kit ELISA, Slc1a1 Kit ELISA
    Sujet

    Gene Name: Excitatory Amino Acid Transporter 3

    Gene Aliases: SLC1A1, EAAC1, Solute Carrier 1 Member 1, Sodium-dependent glutamate/aspartate transporter 3, Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter

    ID gène
    6505
    UniProt
    P43005
    Pathways
    Dicarboxylic Acid Transport
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