HBEGF Kit CLIA
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- Antigène Voir toutes HBEGF Kits CLIA
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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Reactivité
- Rat
- Méthode de détection
- Chemiluminescent
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.62 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.62 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
- Sensibilité
- 0.55 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 - 3 h
- Plaque
- Pre-coated
- Protocole
- The microplate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) level in the sample or standard.,
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes HBEGF Kits CLIA
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
- Autre désignation
- Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF Produits)
- Synonymes
- hbegf Kit CLIA, zgc:165443 Kit CLIA, HBEGF Kit CLIA, zgc:175091 Kit CLIA, DTR Kit CLIA, DTS Kit CLIA, DTSF Kit CLIA, HEGFL Kit CLIA, Dtr Kit CLIA, GFHB Kit CLIA, Hb-egf Kit CLIA, Hegfl Kit CLIA, AW047313 Kit CLIA, Dts Kit CLIA, HB-EGF Kit CLIA, heparin-binding EGF-like growth factor b Kit CLIA, heparin binding EGF like growth factor Kit CLIA, Heparin-binding EGF-like growth factor Kit CLIA, heparin-binding EGF-like growth factor a Kit CLIA, heparin-binding EGF-like growth factor Kit CLIA, hbegfb Kit CLIA, HBEGF Kit CLIA, hbegf Kit CLIA, hbegfa Kit CLIA, Hbegf Kit CLIA
- Sujet
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Gene Name: Heparin Binding Epidermal Growth Factor Like Growth Factor
Gene Aliases: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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