HMGB1 Kit CLIA

N° du produit ABIN5655131

Détail du produit HMGB1 Kit CLIA

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.

Sensibilité: 5.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMG1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the High Mobility Group Protein 1 (HMG1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High Mobility Group Protein 1 (HMGB1 Produits)

Synonymes: HMG1 Kit CLIA, HMG3 Kit CLIA, SBP-1 Kit CLIA, DEF Kit CLIA, HMG-1 Kit CLIA, Hmg1 Kit CLIA, amphoterin Kit CLIA, p30 Kit CLIA, hmgb1 Kit CLIA, ik:tdsubc_1a5 Kit CLIA, wu:fb23c02 Kit CLIA, xx:tdsubc_1a5 Kit CLIA, zgc:56110 Kit CLIA, zgc:77104 Kit CLIA, hmg-1 Kit CLIA, hmg3 Kit CLIA, sbp-1 Kit CLIA, hmg1 Kit CLIA, HMGB1 Kit CLIA, Ac2-008 Kit CLIA, high mobility group box 1 Kit CLIA, high-mobility group box 1 Kit CLIA, high mobility group box 1a Kit CLIA, high mobility group box 1 L homeolog Kit CLIA, high mobility group protein B1 Kit CLIA, HMGB1 Kit CLIA, Hmgb1 Kit CLIA, hmgb1 Kit CLIA, hmgb1a Kit CLIA, hmgb1.L Kit CLIA, LOC100359149 Kit CLIA

Sujet:

Gene Name: High Mobility Group Protein 1

Gene Aliases: HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein

ID gène: 3146

UniProt: P09429

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome