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HMGB1 Kit ELISA

HMGB1 Reactivité: Souris Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
N° du produit ABIN5655133
  • Antigène Voir toutes HMGB1 Kits ELISA
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    Reactivité
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    46.88 pg/mL - 3000 pg/mL
    Seuil minimal de détection
    46.88 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.
    Sensibilité
    18.29 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Protein 1 (HMG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Protein 1 (HMG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes HMGB1 Kits ELISA
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    Autre désignation
    High Mobility Group Protein 1 (HMGB1 Produits)
    Synonymes
    HMG1 Kit ELISA, HMG3 Kit ELISA, SBP-1 Kit ELISA, DEF Kit ELISA, HMG-1 Kit ELISA, Hmg1 Kit ELISA, amphoterin Kit ELISA, p30 Kit ELISA, hmgb1 Kit ELISA, ik:tdsubc_1a5 Kit ELISA, wu:fb23c02 Kit ELISA, xx:tdsubc_1a5 Kit ELISA, zgc:56110 Kit ELISA, zgc:77104 Kit ELISA, hmg-1 Kit ELISA, hmg3 Kit ELISA, sbp-1 Kit ELISA, hmg1 Kit ELISA, HMGB1 Kit ELISA, Ac2-008 Kit ELISA, high mobility group box 1 Kit ELISA, high-mobility group box 1 Kit ELISA, high mobility group box 1a Kit ELISA, high mobility group box 1 L homeolog Kit ELISA, high mobility group protein B1 Kit ELISA, HMGB1 Kit ELISA, Hmgb1 Kit ELISA, hmgb1 Kit ELISA, hmgb1a Kit ELISA, hmgb1.L Kit ELISA, LOC100359149 Kit ELISA
    Sujet

    Gene Name: High Mobility Group Protein 1

    Gene Aliases: HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein

    ID gène
    100862258
    UniProt
    P63158
    Pathways
    Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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