IKBKB Kit ELISA

N° du produit ABIN5655361

Détail du produit IKBKB Kit ELISA

Antigène: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). No significant cross-reactivity or interference between Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Autre désignation: Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IKBKB Produits)

Synonymes: ikk-beta Kit ELISA, ikk2 Kit ELISA, ikkb Kit ELISA, ikkbeta Kit ELISA, IKBKB Kit ELISA, IKKB Kit ELISA, CG4201 Kit ELISA, DIK Kit ELISA, DLAK Kit ELISA, DmIKK Kit ELISA, DmIKKBeta Kit ELISA, DmIKKb Kit ELISA, DmIKKbeta Kit ELISA, DmIkkbeta Kit ELISA, Dmel\\CG4201 Kit ELISA, Dmikkb Kit ELISA, Dmikkbeta Kit ELISA, IK Kit ELISA, IKK Kit ELISA, IKK-beta Kit ELISA, IKKb Kit ELISA, IKKbeta Kit ELISA, IRD-5 Kit ELISA, IRD5 Kit ELISA, Ik Kit ELISA, Ikkb Kit ELISA, Ikkbeta Kit ELISA, Ird-5 Kit ELISA, Ird5 Kit ELISA, LRK Kit ELISA, Lak Kit ELISA, anon-89Bd Kit ELISA, dIKK Kit ELISA, dIKK-beta Kit ELISA, dIKKbeta Kit ELISA, dLak Kit ELISA, dmIKKbeta Kit ELISA, ikk Kit ELISA, ird Kit ELISA, ird5/DmIkk[beta] Kit ELISA, IKK2 Kit ELISA, NFKBIKB Kit ELISA, AIM-1 Kit ELISA, AI132552 Kit ELISA, IKK-2 Kit ELISA, IKK[b] Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog Kit ELISA, inhibitor of nuclear factor kappa B kinase subunit beta Kit ELISA, I-kappaB kinase beta Kit ELISA, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta Kit ELISA, inhibitor of kappaB kinase beta Kit ELISA, ikbkb.L Kit ELISA, IKBKB Kit ELISA, ikbkb Kit ELISA, IKKbeta Kit ELISA, Ikbkb Kit ELISA

Sujet:

Gene Name: Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta

Gene Aliases: IKK2, NFKBIKB, IKK-beta, IKKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta

ID gène: 16150

UniProt: O88351

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins