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IL1R1 Kit ELISA

IL1R1 Reactivité: Souris Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5655706
  • Antigène Voir toutes IL1R1 Kits ELISA
    IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
    Reactivité
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Type I (IL1R1). No significant cross-reactivity or interference between Interleukin 1 Receptor Type I (IL1R1) and analogues was observed.
    Sensibilité
    14.8 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Type I (IL1R1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Type I (IL1R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Type I (IL1R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Type I (IL1R1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Type I (IL1R1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Type I (IL1R1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes IL1R1 Kits ELISA
    IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))
    Autre désignation
    Interleukin 1 Receptor Type I (IL1R1 Produits)
    Synonymes
    CD121A Kit ELISA, D2S1473 Kit ELISA, IL-1R-alpha Kit ELISA, IL1R Kit ELISA, IL1RA Kit ELISA, P80 Kit ELISA, CD121a Kit ELISA, CD121b Kit ELISA, IL-iR Kit ELISA, Il1r-1 Kit ELISA, DER4 Kit ELISA, Fit-1 Kit ELISA, Ly84 Kit ELISA, ST2L Kit ELISA, St2 Kit ELISA, St2-rs1 Kit ELISA, T1 Kit ELISA, T1/ST2 Kit ELISA, interleukin 1 receptor type 1 Kit ELISA, interleukin 1 receptor, type I Kit ELISA, interleukin 1 receptor-like 1 Kit ELISA, IL1R1 Kit ELISA, Il1r1 Kit ELISA, Il1rl1 Kit ELISA
    Sujet

    Gene Name: Interleukin 1 Receptor Type I

    Gene Aliases: CD121a, CD121-A, IL1-R1, IL1R, IL1RA, P80, CD121 Antigen-Like Family Member A, Interleukin-1 Receptor Alpha

    ID gène
    16177
    UniProt
    P13504
    Pathways
    Signalisation NF-kappaB, Carbohydrate Homeostasis, Cancer Immune Checkpoints
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