IL12A Kit ELISA

N° du produit ABIN5655755

Détail du produit IL12A Kit ELISA

Antigène: IL12A (Interleukin 12 alpha (IL12A))

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 12A (IL12A). No significant cross-reactivity or interference between Interleukin 12A (IL12A) and analogues was observed.

Sensibilité: 3.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12A (IL12A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12A (IL12A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12A (IL12A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12A (IL12A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12A (IL12A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IL12A (Interleukin 12 alpha (IL12A))

Autre désignation: Interleukin 12A (IL12A Produits)

Synonymes: CLMF Kit ELISA, IL-12A Kit ELISA, NFSK Kit ELISA, NKSF1 Kit ELISA, P35 Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12p35 Kit ELISA, Il-12a Kit ELISA, Ll12a Kit ELISA, p35 Kit ELISA, IL12p35 Kit ELISA, Il-12 p35 Kit ELISA, IL12p40 Kit ELISA, IL12 Kit ELISA, IL-12 p35 Kit ELISA, interleukin 12A Kit ELISA, interleukin 12B Kit ELISA, interleukin 12b Kit ELISA, interleukin 12a Kit ELISA, Interleukin 12 level Kit ELISA, IL12A Kit ELISA, IL12B Kit ELISA, Il12b Kit ELISA, Il12a Kit ELISA, IL12 Kit ELISA, il12a Kit ELISA

Sujet:

Gene Name: Interleukin 12A

Gene Aliases: p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation