KIR3DL1 Kit ELISA

N° du produit ABIN5656219

Détail du produit KIR3DL1 Kit ELISA

Antigène: KIR3DL1 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 1 (KIR3DL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). No significant cross-reactivity or interference between Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) and analogues was observed.

Sensibilité: 0.051 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KIR3DL1 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 1 (KIR3DL1))

Autre désignation: Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1 Produits)

Synonymes: KIR3DL1 Kit ELISA, KIR3DL3 Kit ELISA, CD158E1 Kit ELISA, KIR Kit ELISA, KIR3DL1/S1 Kit ELISA, NKAT3 Kit ELISA, NKB1 Kit ELISA, NKB1B Kit ELISA, Kirl1 Kit ELISA, Kirl2 Kit ELISA, Krl1 Kit ELISA, killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1 Kit ELISA, uncharacterized LOC100437736 Kit ELISA, killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 1 Kit ELISA, KIR3DL1 Kit ELISA, LOC100437736 Kit ELISA, Kir3dl1 Kit ELISA

Sujet:

Gene Name: Killer Cell Immunoglobulin Like Receptor 3DL1

Gene Aliases: CD158E, HLA-BW4-specific inhibitory NK cell receptor, CD158 antigen-like family member E, Natural killer-associated transcript 3, p70 NK receptor CL-2/CL-11

ID gène: 3811

UniProt: P43629