Lactoferrin Kit CLIA
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- Antigène Voir toutes Lactoferrin (LTF) Kits CLIA
- Lactoferrin (LTF) (Lactotransferrin (LTF))
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Reactivité
- Boeuf (Vache)
- Méthode de détection
- Chemiluminescent
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 1.17 ng/mL - 300 ng/mL
- Seuil minimal de détection
- 1.17 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Saliva, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Lactoferrin (LTF). No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.
- Sensibilité
- 0.14 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 - 3 h
- Plaque
- Pre-coated
- Protocole
- The microplate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lactoferrin (LTF) level in the sample or standard.,
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Lactoferrin (LTF) Kits CLIA
- Lactoferrin (LTF) (Lactotransferrin (LTF))
- Autre désignation
- Lactoferrin (LTF Produits)
- Synonymes
- LTF Kit CLIA, GIG12 Kit CLIA, HLF2 Kit CLIA, LF Kit CLIA, Lf Kit CLIA, Csp82 Kit CLIA, MMS10R Kit CLIA, Ms10r Kit CLIA, PLF Kit CLIA, TFEW Kit CLIA, conalbumin Kit CLIA, lactotransferrin Kit CLIA, transferrin Kit CLIA, transferrin (ovotransferrin) Kit CLIA, LTF Kit CLIA, CpipJ_CPIJ000114 Kit CLIA, TF Kit CLIA, Ltf Kit CLIA
- Sujet
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Gene Name: Lactoferrin
Gene Aliases: HLF2, LF, GIG12, Lactotransferrin, Growth-inhibiting protein 12, Talalactoferrin, Lfcin-H, Kaliocin-1
- ID gène
- 280846
- UniProt
- P24627
- Pathways
- Transition Metal Ion Homeostasis
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