Lamin A/C Kit ELISA

N° du produit ABIN5656297

Détail du produit Lamin A/C Kit ELISA

Antigène: Lamin A/C (LMNA)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lamin A/C (LMNA). No significant cross-reactivity or interference between Lamin A/C (LMNA) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin A/C (LMNA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin A/C (LMNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin A/C (LMNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin A/C (LMNA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lamin A/C (LMNA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lamin A/C (LMNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Lamin A/C (LMNA)

Autre désignation: Lamin A/C (LMNA Produits)

Synonymes: CDCD1 Kit ELISA, CDDC Kit ELISA, CMD1A Kit ELISA, CMT2B1 Kit ELISA, EMD2 Kit ELISA, FPL Kit ELISA, FPLD Kit ELISA, FPLD2 Kit ELISA, HGPS Kit ELISA, IDC Kit ELISA, LDP1 Kit ELISA, LFP Kit ELISA, LGMD1B Kit ELISA, LMN1 Kit ELISA, LMNC Kit ELISA, LMNL1 Kit ELISA, PRO1 Kit ELISA, Dhe Kit ELISA, 2459 Kit ELISA, 74/76 Kit ELISA, CG6944 Kit ELISA, D5 Kit ELISA, DM[[O]] Kit ELISA, Dm Kit ELISA, Dm(0) Kit ELISA, Dm0 Kit ELISA, Dm1 Kit ELISA, Dm2 Kit ELISA, DmLamin Kit ELISA, DmO Kit ELISA, Dm[[0]] Kit ELISA, Dm[[1]] Kit ELISA, Dm[[2]] Kit ELISA, Dm[[mit]] Kit ELISA, Dm[[o]] Kit ELISA, Dmel\\CG6944 Kit ELISA, Dmo Kit ELISA, LAM Kit ELISA, Lam Dm[[0]] Kit ELISA, Lam(Dm0) Kit ELISA, LamDm[[0]] Kit ELISA, LamDm[[o]] Kit ELISA, Lam[[Dm0]] Kit ELISA, Lamin Dm[[0]] Kit ELISA, jf27 Kit ELISA, l(2)04643 Kit ELISA, l(2)25Ec Kit ELISA, l(2)gdh-7 Kit ELISA, l(2)gdh7 Kit ELISA, l(2)jf27 Kit ELISA, lam Kit ELISA, lamDm0 Kit ELISA, lamDm[[0]] Kit ELISA, lamin Kit ELISA, lamin Dm0 Kit ELISA, lamin Dm[[0]] Kit ELISA, misg Kit ELISA, nlam Kit ELISA, cb948 Kit ELISA, fk66d12 Kit ELISA, wu:fk66d12 Kit ELISA, lmna-A Kit ELISA, fpl Kit ELISA, idc Kit ELISA, lfp Kit ELISA, cddc Kit ELISA, emd2 Kit ELISA, fpld Kit ELISA, hgps Kit ELISA, ldp1 Kit ELISA, lmn1 Kit ELISA, lmnc Kit ELISA, pro1 Kit ELISA, cdcd1 Kit ELISA, cmd1a Kit ELISA, cmt2b1 Kit ELISA, lgmd1b Kit ELISA, Lamin-A Kit ELISA, lamin-L(III)-like Kit ELISA, lamin A/C Kit ELISA, lamin A Kit ELISA, Lamin Kit ELISA, lamin A/C L homeolog Kit ELISA, lamin-L(III)-like Kit ELISA, LMNA Kit ELISA, Lmna Kit ELISA, Lam Kit ELISA, lmna Kit ELISA, lmna.L Kit ELISA, LMINA Kit ELISA

Sujet:

Gene Name: Lamin A/C

Gene Aliases: CDCD1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1, Cardiomyopathy Dilated 1A, Limb Girdle Muscular Dystrophy 1B, Progeria 1

ID gène: 4000

UniProt: P02545

Pathways: Apoptose, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus