Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

PSMB8 Kit ELISA

PSMB8 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5656323
  • Antigène Voir toutes PSMB8 Kits ELISA
    PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))
    Reactivité
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Large Multifunctional Peptidase 7 (LMP7). No significant cross-reactivity or interference between Large Multifunctional Peptidase 7 (LMP7) and analogues was observed.
    Sensibilité
    0.117 ng/mL
    Featured
    Discover our best selling PSMB8 Kit ELISA
    Top Product
    Discover our top product PSMB8 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Multifunctional Peptidase 7 (LMP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Large Multifunctional Peptidase 7 (LMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Multifunctional Peptidase 7 (LMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Multifunctional Peptidase 7 (LMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Large Multifunctional Peptidase 7 (LMP7) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Large Multifunctional Peptidase 7 (LMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes PSMB8 Kits ELISA
    PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))
    Autre désignation
    Large Multifunctional Peptidase 7 (PSMB8 Produits)
    Synonymes
    ALDD Kit ELISA, D6S216 Kit ELISA, D6S216E Kit ELISA, JMP Kit ELISA, LMP7 Kit ELISA, NKJO Kit ELISA, PSMB5i Kit ELISA, RING10 Kit ELISA, ATP6V0D2 Kit ELISA, lmp7 Kit ELISA, prosome Kit ELISA, macropain Kit ELISA, Lmp-7 Kit ELISA, Lmp7 Kit ELISA, Ring10 Kit ELISA, proteasome subunit beta 8 Kit ELISA, proteasome subunit beta 8A Kit ELISA, proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) Kit ELISA, PSMB8 Kit ELISA, psmb8a Kit ELISA, Psmb8 Kit ELISA
    Sujet

    Gene Name: Large Multifunctional Peptidase 7

    Gene Aliases: PSMB8, RING10, PSMB5i, Beta5i, Proteasome(Prosome,Macropain)Subunit,Beta Type 8, Multicatalytic endopeptidase complex subunit C13, Really interesting new gene 10 protein

    ID gène
    5696
    UniProt
    P28062
    Pathways
    Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
Vous êtes ici:
Support technique