LCAT Kit CLIA

N° du produit ABIN5656328

Détail du produit LCAT Kit CLIA

Antigène: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 2.34 ng/mL - 150 ng/mL

Seuil minimal de détection: 2.34 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lecithin Cholesterol Acyltransferase (LCAT). No significant cross-reactivity or interference between Lecithin Cholesterol Acyltransferase (LCAT) and analogues was observed.

Sensibilité: 0.1 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lecithin Cholesterol Acyltransferase (LCAT) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Autre désignation: Lecithin Cholesterol Acyltransferase (LCAT Produits)

Synonymes: AI046659 Kit CLIA, D8Wsu61e Kit CLIA, MGC82035 Kit CLIA, lcat Kit CLIA, MGC88964 Kit CLIA, LCAT Kit CLIA, lecithin-cholesterol acyltransferase Kit CLIA, lecithin cholesterol acyltransferase Kit CLIA, lecithin-cholesterol acyltransferase L homeolog Kit CLIA, solute carrier family 12 member 4 Kit CLIA, fragile site, aphidicolin type, common, fra(13)(q13.2) Kit CLIA, LCAT Kit CLIA, Lcat Kit CLIA, lcat.L Kit CLIA, lcat Kit CLIA, SLC12A4 Kit CLIA, FRA13A Kit CLIA

Sujet:

Gene Name: Lecithin Cholesterol Acyltransferase

Gene Aliases: phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase

ID gène: 3931

UniProt: P04180

Pathways: Lipid Metabolism