SERPINB1 Kit ELISA

N° du produit ABIN5656409

Détail du produit SERPINB1 Kit ELISA

Antigène: SERPINB1 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 1 (SERPINB1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Leukocyte Elastase Inhibitor (LEI). No significant cross-reactivity or interference between Leukocyte Elastase Inhibitor (LEI) and analogues was observed.

Sensibilité: 6.4 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Elastase Inhibitor (LEI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Elastase Inhibitor (LEI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Elastase Inhibitor (LEI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukocyte Elastase Inhibitor (LEI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukocyte Elastase Inhibitor (LEI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukocyte Elastase Inhibitor (LEI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SERPINB1 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 1 (SERPINB1))

Autre désignation: Leukocyte Elastase Inhibitor (SERPINB1 Produits)

Synonymes: ELANH2 Kit ELISA, zgc:91981 Kit ELISA, si:dkey-177p2.11 Kit ELISA, si:dkey-177p2.12 Kit ELISA, EI Kit ELISA, LEI Kit ELISA, M/NEI Kit ELISA, MNEI Kit ELISA, PI-2 Kit ELISA, PI2 Kit ELISA, elanh2 Kit ELISA, lei Kit ELISA, m/nei Kit ELISA, mnei Kit ELISA, pi2 Kit ELISA, serpin peptidase inhibitor, clade B (ovalbumin), member 1 Kit ELISA, serpin family B member 1 Kit ELISA, serpin family B member 1 L homeolog Kit ELISA, SERPINB1 Kit ELISA, serpinb1 Kit ELISA, serpinb1.L Kit ELISA

Sujet:

Gene Name: Leukocyte Elastase Inhibitor

Gene Aliases: SERPINB1, EI, ELANH2, LEI, M/NEI, MNEI, PI2, Serpin Peptidase Inhibitor Clade B Member 1, Anti-Elastase, Monocyte/Neutrophil Elastase Inhibitor

ID gène: 66222

UniProt: Q9D154