MMP 9 Kit CLIA

N° du produit ABIN5656795

Détail du produit MMP 9 Kit CLIA

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 117.19 pg/mL - 30000 pg/mL

Seuil minimal de détection: 117.19 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9). No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.

Sensibilité: 14.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 9 (MMP9) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Autre désignation: Matrix Metalloproteinase 9 (MMP9 Produits)

Synonymes: CLG4B Kit CLIA, GELB Kit CLIA, MANDP2 Kit CLIA, MMP-9 Kit CLIA, AW743869 Kit CLIA, B/MMP9 Kit CLIA, Clg4b Kit CLIA, pro-MMP-9 Kit CLIA, mmp-9 Kit CLIA, fgMMP-9 Kit CLIA, mmp9 Kit CLIA, ZFMMP-9 Kit CLIA, wu:fb02g06 Kit CLIA, wu:fb07b05 Kit CLIA, wu:fi98c09 Kit CLIA, wu:fj05a08 Kit CLIA, zgc:64165 Kit CLIA, clg4b Kit CLIA, gelb Kit CLIA, mandp2 Kit CLIA, matrix metallopeptidase 9 Kit CLIA, collagenase Kit CLIA, matrix metalloproteinase 9 Kit CLIA, matrix metallopeptidase 9 S homeolog Kit CLIA, MMP9 Kit CLIA, RB3913 Kit CLIA, BPSS0666 Kit CLIA, Sbal_3732 Kit CLIA, Bcer98_0486 Kit CLIA, Shew185_0630 Kit CLIA, Shal_3056 Kit CLIA, Sbal195_0657 Kit CLIA, Lbys_3550 Kit CLIA, Palpr_2084 Kit CLIA, Mmp9 Kit CLIA, mmp9 Kit CLIA, mmp9.S Kit CLIA

Sujet:

Gene Name: Matrix Metalloproteinase 9

Gene Aliases: GELB, Gelatinase B, CLG4B, CLG4-B, 92 KDa Gelatinase, 92 kDa Type IV Collagenase

ID gène: 17395

UniProt: P41245

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events