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MMP 9 Kit ELISA

MMP9 Reactivité: Porc Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656797
  • Antigène Voir toutes MMP 9 (MMP9) Kits ELISA
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    Reactivité
    • 12
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Porc
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    3.12 ng/mL - 200 ng/mL
    Seuil minimal de détection
    3.12 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9). No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.
    Sensibilité
    1.38 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 9 (MMP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 9 (MMP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MMP 9 (MMP9) Kits ELISA
    MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))
    Autre désignation
    Matrix Metalloproteinase 9 (MMP9 Produits)
    Synonymes
    CLG4B Kit ELISA, GELB Kit ELISA, MANDP2 Kit ELISA, MMP-9 Kit ELISA, AW743869 Kit ELISA, B/MMP9 Kit ELISA, Clg4b Kit ELISA, pro-MMP-9 Kit ELISA, mmp-9 Kit ELISA, fgMMP-9 Kit ELISA, mmp9 Kit ELISA, ZFMMP-9 Kit ELISA, wu:fb02g06 Kit ELISA, wu:fb07b05 Kit ELISA, wu:fi98c09 Kit ELISA, wu:fj05a08 Kit ELISA, zgc:64165 Kit ELISA, clg4b Kit ELISA, gelb Kit ELISA, mandp2 Kit ELISA, matrix metallopeptidase 9 Kit ELISA, collagenase Kit ELISA, matrix metalloproteinase 9 Kit ELISA, matrix metallopeptidase 9 S homeolog Kit ELISA, MMP9 Kit ELISA, RB3913 Kit ELISA, BPSS0666 Kit ELISA, Sbal_3732 Kit ELISA, Bcer98_0486 Kit ELISA, Shew185_0630 Kit ELISA, Shal_3056 Kit ELISA, Sbal195_0657 Kit ELISA, Lbys_3550 Kit ELISA, Palpr_2084 Kit ELISA, Mmp9 Kit ELISA, mmp9 Kit ELISA, mmp9.S Kit ELISA
    Sujet

    Gene Name: Matrix Metalloproteinase 9

    Gene Aliases: GELB, Gelatinase B, CLG4B, CLG4-B, 92 KDa Gelatinase, 92 kDa Type IV Collagenase

    Pathways
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events
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