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Neuroserpin Kit ELISA

SERPINI1 Reactivité: Humain Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum
N° du produit ABIN5657332
  • Antigène Voir toutes Neuroserpin (SERPINI1) Kits ELISA
    Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.781 ng/mL - 50 ng/mL
    Seuil minimal de détection
    0.781 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Neuroserpin (NSP). No significant cross-reactivity or interference between Neuroserpin (NSP) and analogues was observed.
    Sensibilité
    0.34 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroserpin (NSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroserpin (NSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroserpin (NSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroserpin (NSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroserpin (NSP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroserpin (NSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Neuroserpin (SERPINI1) Kits ELISA
    Neuroserpin (SERPINI1) (serpin Peptidase Inhibitor, Clade I (neuroserpin), Member 1 (SERPINI1))
    Autre désignation
    Neuroserpin (SERPINI1 Produits)
    Synonymes
    PI12 Kit ELISA, neuroserpin Kit ELISA, AI837402 Kit ELISA, Ns Kit ELISA, PI-12 Kit ELISA, Spi17 Kit ELISA, CG9453 Kit ELISA, Dmel\\CG9453 Kit ELISA, Serp2 Kit ELISA, Sp4 Kit ELISA, Spn4 Kit ELISA, Spn4A Kit ELISA, dSerp2 Kit ELISA, sp4 Kit ELISA, spn4 Kit ELISA, raPIT5a Kit ELISA, SERPINI1 Kit ELISA, pi12 Kit ELISA, serpini1l Kit ELISA, si:ch211-167c22.4 Kit ELISA, serpin family I member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade I, member 1 Kit ELISA, Serpin 42Da Kit ELISA, serpin peptidase inhibitor, clade I (neuroserpin), member 1 Kit ELISA, serpin family I member 1 L homeolog Kit ELISA, SERPINI1 Kit ELISA, Serpini1 Kit ELISA, Spn42Da Kit ELISA, serpini1 Kit ELISA, serpini1.L Kit ELISA
    Sujet

    Gene Name: Neuroserpin

    Gene Aliases: SERPINI1, PI12, Serpin Peptidase Inhibitor Clade I Member 1, Peptidase inhibitor 12

    ID gène
    5274
    UniProt
    Q99574
    Pathways
    Regulation of Hormone Metabolic Process
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