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PDGFB Kit CLIA

PDGFB Reactivité: Humain Chemiluminescent Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma
N° du produit ABIN5657917
  • Antigène Voir toutes PDGFB Kits CLIA
    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
    Reactivité
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB). No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) and analogues was observed.
    Sensibilité
    1.26 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 - 3 h
    Plaque
    Pre-coated
    Protocole
    The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Derived Growth Factor Subunit B (PDGFB) level in the sample or standard.,
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes PDGFB Kits CLIA
    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
    Autre désignation
    Platelet Derived Growth Factor Subunit B (PDGFB Produits)
    Synonymes
    PDGF-2 Kit CLIA, PDGF2 Kit CLIA, SIS Kit CLIA, SSV Kit CLIA, c-sis Kit CLIA, pdgf2 Kit CLIA, sis Kit CLIA, si:ch211-79m20.1 Kit CLIA, si:dkey-261d11.1 Kit CLIA, PDGF-B Kit CLIA, Sis Kit CLIA, platelet derived growth factor subunit B Kit CLIA, platelet-derived growth factor beta polypeptide Kit CLIA, platelet derived growth factor subunit B S homeolog Kit CLIA, platelet-derived growth factor beta polypeptide a Kit CLIA, platelet derived growth factor, B polypeptide Kit CLIA, PDGFB Kit CLIA, pdgfb.S Kit CLIA, pdgfba Kit CLIA, Pdgfb Kit CLIA
    Sujet

    Gene Name: Platelet Derived Growth Factor Subunit B

    Gene Aliases: PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral(v-sis)Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin

    ID gène
    5155
    UniProt
    P01127
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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