PARP1 Kit CLIA

N° du produit ABIN5657973

Détail du produit PARP1 Kit CLIA

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Poly ADP Ribose Polymerase (PARP). No significant cross-reactivity or interference between Poly ADP Ribose Polymerase (PARP) and analogues was observed.

Sensibilité: 0.11 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Poly ADP Ribose Polymerase (PARP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Poly ADP Ribose Polymerase (PARP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Poly ADP Ribose Polymerase (PARP) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poly ADP Ribose Polymerase (PARP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: Poly ADP Ribose Polymerase (PARP1 Produits)

Synonymes: ADPRT Kit CLIA, ADPRT 1 Kit CLIA, ADPRT1 Kit CLIA, ARTD1 Kit CLIA, PARP Kit CLIA, PARP-1 Kit CLIA, PPOL Kit CLIA, pADPRT-1 Kit CLIA, Adprt Kit CLIA, Parp-1 Kit CLIA, 5830444G22Rik Kit CLIA, AI893648 Kit CLIA, Adprp Kit CLIA, Adprt1 Kit CLIA, C80510 Kit CLIA, parp-1 Kit CLIA, sPARP-1 Kit CLIA, BEST:LD21673 Kit CLIA, CG17685 Kit CLIA, CG17696 Kit CLIA, CG17718 Kit CLIA, CG40411 Kit CLIA, D.PARP Kit CLIA, Dm.pARTa Kit CLIA, Dmel\\CG40411 Kit CLIA, LD21673.3prime Kit CLIA, PARP1 Kit CLIA, dPARP Kit CLIA, parp Kit CLIA, adprt1 Kit CLIA, padprt-1 Kit CLIA, ppol Kit CLIA, si:dkey-206f10.3 Kit CLIA, wu:fc60f12 Kit CLIA, zgc:110092 Kit CLIA, poly(ADP-ribose) polymerase 1 Kit CLIA, poly (ADP-ribose) polymerase 1 Kit CLIA, poly (ADP-ribose) polymerase family, member 1 Kit CLIA, Poly-(ADP-ribose) polymerase Kit CLIA, poly(ADP-ribose) polymerase 1 L homeolog Kit CLIA, PARP1 Kit CLIA, Parp1 Kit CLIA, Parp Kit CLIA, parp1.L Kit CLIA, parp1 Kit CLIA

Sujet:

Gene Name: Poly ADP Ribose Polymerase

Gene Aliases: PARP1, ADPRT, ADPRT1, PPOL, pADPRT-1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1

ID gène: 142

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location