NR1I2 Kit ELISA

N° du produit ABIN5658003

Détail du produit NR1I2 Kit ELISA

Antigène: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Pregnane X Receptor (PXR). No significant cross-reactivity or interference between Pregnane X Receptor (PXR) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnane X Receptor (PXR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pregnane X Receptor (PXR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnane X Receptor (PXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnane X Receptor (PXR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pregnane X Receptor (PXR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pregnane X Receptor (PXR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Autre désignation: Pregnane X Receptor (NR1I2 Produits)

Synonymes: BXR Kit ELISA, ONR1 Kit ELISA, PAR Kit ELISA, PAR1 Kit ELISA, PAR2 Kit ELISA, PARq Kit ELISA, PRR Kit ELISA, PXR Kit ELISA, SAR Kit ELISA, SXR Kit ELISA, PXR.1 Kit ELISA, PXR.2 Kit ELISA, PXR1 Kit ELISA, mPXR Kit ELISA, bxr Kit ELISA, nr1i2 Kit ELISA, onr1 Kit ELISA, xonr1 Kit ELISA, nuclear receptor subfamily 1 group I member 2 Kit ELISA, nuclear receptor subfamily 1, group I, member 2 Kit ELISA, nuclear receptor subfamily 1 group I member 2 S homeolog Kit ELISA, NR1I2 Kit ELISA, Nr1i2 Kit ELISA, nr1i2.S Kit ELISA, nr1i2 Kit ELISA

Sujet:

Gene Name: Pregnane X Receptor

Gene Aliases: NR1I2, SAR, PAR1, PAR2, PRR, BXR, ONR1, PAR, PARq, SXR, Nuclear Receptor Subfamily 1,Group I,Member 2, Steroid and xenobiotic receptor, Pregnane X receptor

ID gène: 84385

UniProt: Q9R1A7

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway