PSMA1 Kit ELISA
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- Antigène Voir toutes PSMA1 Kits ELISA
- PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Alpha Type 1 (PSMa1). No significant cross-reactivity or interference between Proteasome Subunit Alpha Type 1 (PSMa1) and analogues was observed.
- Sensibilité
- 0.067 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 1 (PSMa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 1 (PSMa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 1 (PSMa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteasome Subunit Alpha Type 1 (PSMa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 1 (PSMa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 1 (PSMa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes PSMA1 Kits ELISA
- PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))
- Autre désignation
- Proteasome Subunit Alpha Type 1 (PSMA1 Produits)
- Synonymes
- PSMA1 Kit ELISA, pros30 Kit ELISA, DDBDRAFT_0204226 Kit ELISA, DDBDRAFT_0214956 Kit ELISA, DDB_0204226 Kit ELISA, DDB_0214956 Kit ELISA, C2 Kit ELISA, HC2 Kit ELISA, Pros-30 Kit ELISA, alpha-type Kit ELISA, NU Kit ELISA, PROS30 Kit ELISA, CC2 Kit ELISA, zgc:92726 Kit ELISA, 143314_at Kit ELISA, Alpha-6 Kit ELISA, CG4904 Kit ELISA, Dmel\CG4904 Kit ELISA, PROS-35 Kit ELISA, PROS-Dm35 Kit ELISA, Pros-35 Kit ELISA, Pros-Dm35 Kit ELISA, Prosalpha6 Kit ELISA, alpha6 Kit ELISA, alpha_dm Kit ELISA, anon-SAGE:Wang-116 Kit ELISA, pros 35 Kit ELISA, pros35 Kit ELISA, proteasome subunit alpha 1 Kit ELISA, proteasome subunit alpha type 1 Kit ELISA, 20S proteasome subunit C2 Kit ELISA, proteasome (prosome, macropain) subunit, alpha type 1 Kit ELISA, proteasome subunit alpha 1 L homeolog Kit ELISA, Proteasome alpha6 subunit Kit ELISA, PSMA1 Kit ELISA, psma1 Kit ELISA, CNB05540 Kit ELISA, psmA1 Kit ELISA, Psma1 Kit ELISA, psma1.L Kit ELISA, Prosalpha6 Kit ELISA
- Sujet
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Gene Name: Proteasome Subunit Alpha Type 1
Gene Aliases: HC2, NU, PROS30, Proteasome(Prosome,Macropain)Subunit,Alpha Type,1, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome nu chain
- ID gène
- 5682
- UniProt
- P25786
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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