AKT3 Kit ELISA
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- Antigène Voir toutes AKT3 Kits ELISA
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 1.56 ng/mL - 100 ng/mL
- Seuil minimal de détection
- 1.56 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Protein Kinase B Gamma (PKBg). No significant cross-reactivity or interference between Protein Kinase B Gamma (PKBg) and analogues was observed.
- Sensibilité
- 0.56 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Gamma (PKBg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Gamma (PKBg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Gamma (PKBg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Gamma (PKBg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase B Gamma (PKBg) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase B Gamma (PKBg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes AKT3 Kits ELISA
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- Autre désignation
- Protein Kinase B Gamma (AKT3 Produits)
- Synonymes
- MPPH Kit ELISA, PKB-GAMMA Kit ELISA, PKBG Kit ELISA, PRKBG Kit ELISA, RAC-PK-gamma Kit ELISA, RAC-gamma Kit ELISA, STK-2 Kit ELISA, AI851531 Kit ELISA, D930002M15Rik Kit ELISA, Nmf350 Kit ELISA, Pkbg Kit ELISA, AKT serine/threonine kinase 3 Kit ELISA, thymoma viral proto-oncogene 3 Kit ELISA, v-akt murine thymoma viral oncogene homolog 3b Kit ELISA, AKT3 Kit ELISA, Akt3 Kit ELISA, akt3b Kit ELISA
- Sujet
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Gene Name: Protein Kinase B Gamma
Gene Aliases: PKBG, PRKB-G, AKT3, RAC-PK-Gamma, RAC-Gamma, STK2, RAC-Gamma Serine/Threonine-Protein Kinase, V-Akt Murine Thymoma Viral Oncogene Homolog 3
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling
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