PRKG1 Kit ELISA

N° du produit ABIN5658237

Détail du produit PRKG1 Kit ELISA

Antigène: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, cGMP Dependent Type I (PRKG1). No significant cross-reactivity or interference between Protein Kinase, cGMP Dependent Type I (PRKG1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cGMP Dependent Type I (PRKG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, cGMP Dependent Type I (PRKG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cGMP Dependent Type I (PRKG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, cGMP Dependent Type I (PRKG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, cGMP Dependent Type I (PRKG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, cGMP Dependent Type I (PRKG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Autre désignation: Protein Kinase, cGMP Dependent Type I (PRKG1 Produits)

Synonymes: prkg1 Kit ELISA, zgc:63955 Kit ELISA, Pkgi Kit ELISA, cGk1 Kit ELISA, 1 Kit ELISA, PKG Kit ELISA, PRKG1B Kit ELISA, PRKGR1B Kit ELISA, cGK Kit ELISA, cGK 1 Kit ELISA, cGK1 Kit ELISA, cGKI Kit ELISA, cGKI-BETA Kit ELISA, cGKI-alpha Kit ELISA, CGKI Kit ELISA, cGKI-beta Kit ELISA, AW125416 Kit ELISA, Gm19690 Kit ELISA, Prkg1b Kit ELISA, Prkgr1b Kit ELISA, Prkg1beta Kit ELISA, protein kinase, cGMP-dependent, type Ia Kit ELISA, protein kinase, cGMP-dependent, type I Kit ELISA, protein kinase, cGMP-dependent, type 1 Kit ELISA, prkg1a Kit ELISA, PRKG1 Kit ELISA, prkg1 Kit ELISA, Prkg1 Kit ELISA

Sujet:

Gene Name: Protein Kinase, cGMP Dependent Type I

Gene Aliases: CGKI, PGK, PRKG1B, PRKGR1B, cGKI-BETA, cGKI-alpha, cGMP-dependent protein kinase 1