Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) Kit ELISA

Détails pour le produit réf. ABIN5658243, Fournisseur: Connectez-vous pour afficher
Antigène
  • 9630030H21
  • Gadd34
  • Myd116
  • GADD34
  • Peg-3
  • RGD1624209
  • protein phosphatase 1 regulatory subunit 15A
  • protein phosphatase 1, regulatory (inhibitor) subunit 15A
  • protein phosphatase 1, regulatory subunit 15A
  • PPP1R15A
  • Ppp1r15a
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
4
3
1
Type de méthode
Sandwich ELISA
Gamme de detection
0.156 ng/mL - 10 ng/mL
Seuil minimal de détection
0.156 ng/mL
Application
ELISA
Options
Fournisseur
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N° du produit (Fournisseur)
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Type d'échantillon Cell Lysate, Tissue Homogenate
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). No significant cross-reactivity or interference between Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) and analogues was observed.
Sensibilité 0.057 ng/mL
Antigène Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A)
Autre désignation Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A ELISA Kit Extrait)
Sujet

Gene Name: Protein Phosphatase 1, Regulatory Subunit 15A

Gene Aliases: PPP1-R15A, PPP1R15-A, PPP1-R15-A, GADD34, Growth Arrest And DNA-Damage-Inducible 34, Myeloid differentiation primary response protein MyD116 homolog

ID gène 17872
UniProt P17564
Pathways ER-Nucleus Signaling, Unfolded protein response
Commentaires

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test 3 h
Plaque Pre-coated
Protocole The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Précision du teste Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Conseil sur la manipulation The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Stock 4 °C,-20 °C
Stockage commentaire -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Date de péremption 4-8 months
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