P2RX7 Kit CLIA

N° du produit ABIN5658301

Détail du produit P2RX7 Kit CLIA

Antigène: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 20000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). No significant cross-reactivity or interference between Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) and analogues was observed.

Sensibilité: 10.28 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Autre désignation: Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7 Produits)

Synonymes: p2xr7 Kit CLIA, P2RX7 Kit CLIA, P2X7 Kit CLIA, AI467586 Kit CLIA, P2X(7) Kit CLIA, P2X7R Kit CLIA, p2x7 Kit CLIA, purinergic receptor P2X 7 Kit CLIA, purinergic receptor P2X, ligand-gated ion channel, 7 Kit CLIA, P2X purinoceptor 7 Kit CLIA, P2RX7 Kit CLIA, p2rx7 Kit CLIA, LOC100458463 Kit CLIA, P2rx7 Kit CLIA

Sujet:

Gene Name: Purinergic Receptor P2X, Ligand Gated Ion Channel 7

Gene Aliases: P2X7, P2X Purinoceptor 7, ATP receptor, Purinergic receptor

ID gène: 5027

UniProt: Q99572

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis