SHC1 Kit ELISA
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- Antigène Voir toutes SHC1 Kits ELISA
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of SHC-Transforming Protein 1 (SHC1). No significant cross-reactivity or interference between SHC-Transforming Protein 1 (SHC1) and analogues was observed.
- Sensibilité
- 0.117 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHC-Transforming Protein 1 (SHC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SHC-Transforming Protein 1 (SHC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SHC-Transforming Protein 1 (SHC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SHC-Transforming Protein 1 (SHC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SHC-Transforming Protein 1 (SHC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SHC-Transforming Protein 1 (SHC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes SHC1 Kits ELISA
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
- Autre désignation
- SHC-Transforming Protein 1 (SHC1 Produits)
- Synonymes
- SHC1 Kit ELISA, SHC Kit ELISA, SHCA Kit ELISA, P66shc Kit ELISA, si:dkey-246i14.4 Kit ELISA, wu:fc10h03 Kit ELISA, shc Kit ELISA, p66shc Kit ELISA, Shc Kit ELISA, ShcA Kit ELISA, p66 Kit ELISA, SHC adaptor protein 1 Kit ELISA, Shc1p Kit ELISA, squalene--hopene cyclase Kit ELISA, SHC (Src homology 2 domain containing) transforming protein 1 Kit ELISA, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog Kit ELISA, src homology 2 domain-containing transforming protein C1 Kit ELISA, SHC1 Kit ELISA, shc1 Kit ELISA, Shc1 Kit ELISA, shc1.L Kit ELISA
- Sujet
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Gene Name: SHC-Transforming Protein 1
Gene Aliases: SHC, SHCA, p52SHC, p66, p66SHC, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1
- ID gène
- 6464
- UniProt
- P29353
- Pathways
- Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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