STAT1 Kit ELISA

N° du produit ABIN5658794

Détail du produit STAT1 Kit ELISA

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 1 (STAT1). No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 1 (STAT1) and analogues was observed.

Sensibilité: 0.115 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 1 (STAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 1 (STAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Autre désignation: Signal Transducer And Activator Of Transcription 1 (STAT1 Produits)

Synonymes: CANDF7 Kit ELISA, ISGF-3 Kit ELISA, STAT91 Kit ELISA, 2010005J02Rik Kit ELISA, AA408197 Kit ELISA, XStat1 Kit ELISA, STAT4 Kit ELISA, DD6G4-4 Kit ELISA, STAT Kit ELISA, STAT1 Kit ELISA, dZ182H3.3 Kit ELISA, si:by134g18.2 Kit ELISA, si:by51f19.1 Kit ELISA, si:dz182h3.3 Kit ELISA, si:dz199m19.1 Kit ELISA, si:dz73o4.2 Kit ELISA, stat1 Kit ELISA, signal transducer and activator of transcription 1 Kit ELISA, signal transducer and activator of transcription 1 L homeolog Kit ELISA, signal transducer and activator of transcription 1, 91kDa Kit ELISA, signal transducer and activator of transcription 4 Kit ELISA, signal transducer and activator of transcription 1a Kit ELISA, STAT1 Kit ELISA, Stat1 Kit ELISA, stat1.L Kit ELISA, stat4 Kit ELISA, stat1a Kit ELISA

Sujet:

Gene Name: Signal Transducer And Activator Of Transcription 1

Gene Aliases: ISGF-3, STAT91, Transcription Factor ISGF-3 Components p91/p84

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events