SIRT1 Kit CLIA

N° du produit ABIN5658808

Détail du produit SIRT1 Kit CLIA

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Reactivité: Rat

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Sirtuin 1 (SIRT1). No significant cross-reactivity or interference between Sirtuin 1 (SIRT1) and analogues was observed.

Sensibilité: 10.52 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Sirtuin 1 (SIRT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Sirtuin 1 (SIRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Sirtuin 1 (SIRT1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Autre désignation: Sirtuin 1 (SIRT1 Produits)

Synonymes: sirtuin 1 Kit CLIA, AA673258 Kit CLIA, SIR2L1 Kit CLIA, Sir2 Kit CLIA, Sir2a Kit CLIA, Sir2alpha Kit CLIA, sir2l1 Kit CLIA, sirtuin Kit CLIA, SIR2 Kit CLIA, sirtuin 1 Kit CLIA, sirtuin 1 L homeolog Kit CLIA, SIRT1 Kit CLIA, SRT1 Kit CLIA, Sirt1 Kit CLIA, sirt1.L Kit CLIA

Sujet:

Gene Name: Sirtuin 1

Gene Aliases: SIR2L1, Silent Mating Type Information Regulation 2 Homolog 1, NAD-Dependent Deacetylase Sirtuin-1, SIR2-like protein 1, SirtT1 75 kDa fragment

Pathways: Signalisation MAPK, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling